.. _alligator-internal-parameters: AlliGator Exposed Internal Parameters +++++++++++++++++++++++++++++++++++++ AlliGator exposed internal parameters' JSON strings with default values ----------------------------------------------------------------------- + # Folds:1 + # Gates:100 + # Logical Processors Used:8 + 1-Normalize Decay:false + Additional Points:100 + All Image ROIs:false + All Phasor Plot ROIs:false + Available Gate Names:["Gate"] + Average Dataset Sum:false + Average Lifetime Options:{"Use Local IRF ":true,"Use IRF Offset":true,"Use Fixed Offset":false,"Fixed Offset":0,"Baseline %":50} + Background Definition Range:{"Min Gate":0,"Max Gate":8.00000000000000E-10} + Background File:"" + Background File Subtraction:false + Background Pile-up Correction:false + Background Pixel Well Capacity:1020 + Background Scaling Factor:1 + Background Subtraction:false + Backup Calibration Type:"Nearest Valid Calibration" + Baseline Subtraction Method:"Min" + Beep after Script:true + Beep on Error:true + Calibration Lifetime:0 + Calibration Type:"No" + Constant Background:0 + Cumulative Phasor Plot:false + Decay & IRF Parameters:{"Lifetime":1,"IRF Offset":0,"IRF Period":1.25000000000000E-8,"Normalized Decays":false,"IRF Model":"Dirac","IRF Parameter 1":0.1} + Decay Analysis Parameters:{"Type":"Default","Shift":0,"Threshold":0,"Rotate":true} + Decay Graph Active:true + Decay Graph Color Array:[1985280,16711680,6618880] + Decay Shift Parameters:{"Type":"Default","Shift":0,"Threshold":0.1,"Rotate":false} + Define Gates to:"Item 0" + Define Shortest Lifetime as:"Item 0" + Display Parameters:{"# Points":1000,"Ramp Type":"Logarithmic","Type of Series":"1-Exp Decays","Min tau":1.00000000000000E-12,"Max tau":1.00000000000000E-7,"Include 0":true,"Include Infinity":true} + Displayed Fit Parameters:[] + Exponential Fading:true + Export Tabulated Results:false + Exported Gate Image Settings:{"bitness":true," Min Value":-Infinity," Max Value":Infinity} + Extract IRF instead of Decay:false + Extrapolate Decay:false + Fit Options:{"Show Fit Residuals":true,"Fit Model":"1-Exponential","Residual Type":"Standard","Max Decay Percentile":1,"Min Decay Percentile":0,"Show Full Decay":true,"Weights":"1/Variance","Fitting Method":"Least Square","Compute Parameter Uncertainties":true,"Periodic Boundaries":true,"Model Calculation":"Convolution","Period":1.25000000000000E-8,"Termination Criteria":{"Max Iterations":1000,"Max Function Calls":10000,"Max Time":1000,"Function Tolerance":1.00000000000000E-9,"Parameter Tolerance":1.00000000000000E-9,"Gradient Tolerance":1.00000000000000E-9,"Min Lambda":1.00000000000000E-9,"Max Lambda":1000000000,"Initial Tick Count":0},"Offset Resolution":5.00000000000000E-11,"Use Local IRF":false,"Fitting Algorithm":"Levenberg-Marquardt"} + Fit Parameter Constraints:[] + Fold Decay:false + Gate Arithmetic:"None" + Gate Duration:3.00000000000000E-10 + Gate Name:0 + Gate Parameters:{"First Gate Start":0,"Use Gate Center":false,"Gate Width":0,"Gate Step":0,"# Gates":Infinity,"Gate Type":"Square","Edge Width":0,"Top Slope":0,"Reversed Sawtooth?":false,"Analytical Computation":false,"Integration":"Item 0","Tolerance":0.001,"# Steps":1000,"Type of User-defined Function":"IRF","Use Gate Step":false} + Gate Separation:4.00000000000000E-11 + Gate Step:1 + Gates to Keep:{"First":0,"Last":4294967295} + Gates to Skip:{"Start":0,"End":0} + Guess Parameter Names:[] + Guess Parameters:[] + Head Fraction:0.5 + High Count Pixels Rejection Options:{"Background High Threshold Factor":0,"Fixed High Background Threshold":Infinity,"Reject High Count Pixels":false,"High Percentile":0} + Histogram Options:{"Bin Size":NaN,"Min Value":-Infinity,"Max Value":Infinity,"Filter NaN & Infinite Values":true,"Frequency Plot":false,"Align Bins":true,"Bin Location":"Left","Project Outside Values":false,"Max Histogram Size":100000} + Hot Pixel Removal:{"Remove Screamers":false,"Delta":Infinity,"Equalize Histogram":false,"Percentile":0} + IRF Extraction Options:{"Time Constant for IRF Extraction":1.00000000000000E-9,"IRF Order":132,"Fourier Series Smoothing":"None","Noise Threshold":0.1,"Smoothing Width":1,"Fourier Series Truncation":"Automatic","Truncation Order":0,"Save Power Spectrum":false,"Smoothing":true,"Smoothing Parameter":-1,"Smoothing Order":10,"Method":"Direct"} + IRF Optimization Options:{"Search Range":5.00000000000000E-10,"Search Step":1.00000000000000E-12,"Tail threshold (%)":5,"Regularized IRF":false,"Export Plot":true,"Export Data":false,"Smooth Metrics":true,"S-G Side Points":6,"S-G Polynomial Order":3} + IRF Order:1 + IRF Time Constants:[] + Image Binning Options:{"Use Image Binning":false,"X Bin":1,"Y Bin":1} + Image Histogram Active:true + Image Smoothing Options:{"Use Image Smoothing":false,"Kernel Type":"Uniform","Bin":1,"Algorithm":"Rapid"} + Include Loading in Timing:false + Intensity Correction File:"" + Intensity Threshold:500 + Invert Binary Mask:false + Laser Period:1.00000000000000E-8 + Last Run Version:false + Lifetime Map Active:false + Low Count Pixels Rejection Options:{"Background Low Threshold Factor":0,"Fixed Low Background Threshold":20000,"Reject Low Count Pixels":false,"Low Percentile":0} + Max Peak Value:Infinity + Min Peak Value:500 + Operations Order:["Background subtraction","Revert","Smoothen","Straighten","Shift","Extrapolate","Normalize","Fold"] + Peak Threshold:500 + Phasor Frequency:80000000 + Phasor Graph Active:true + Phasor Parameters:{"Harmonic Frequency":80000000,"Use f* = 1/D Frequency":false,"Calibrated Phasor":true,"Calibration Lifetime":0,"Phasor Calculation":"Analytical Formula","SEPL Type":"UC","# Gates":1000,"Square Gate Width":0,"Show Ticks":false} + Phasor Plot Active:true + Phasor Plot Graphics Options:{"UC Color":0,"Show UC Tau Ticks":false,"SEPL Color":7171459,"Show SEPL Tau Ticks":false,"Axes Color":0,"Show (g,s) Ticks":true,"Show UC":true,"Show SEPL":false,"Show Axes":true,"Show UC Tau Labels":false,"Show SEPL Tau Labels":false,"Show (g,s) Labels":true} + Phasor Plot Size:{"H":512,"V":512,"Margin":0.5} + Phasor Plot Smoothing Options:{"Use Phasor Plot Smoothing":false,"Kernel Type":"Uniform","Bin":1,"Algorithm":"Rapid"} + Phasor Ratio Color Map:"Rainbow" + Phasor Ratio Color Map Type:"Interpolate Reference Colors" + Phasor Ratio Color-Coded Quantity:"Phasor Ratio" + Phasor Ratio Decay Range:0.02 + Phasor Ratio Display Range:{"Min":0,"Max":1} + Phasor Ratio Reference Colors:{"Reference 1":6618880,"Reference 2":16711680,"Boundary":9868950} + Phasor Ratio Type:"Item 0" + Pile-up Correction:false + Pixel Well Capacity:1020 + Pixel-Normalize Decay:false + Pixel-level Phasor Computation:false + Python Settings:{"Python Version":"3.9","Use PATH definition":true,"Python.exe Path":""} + ROI Center Color:16711680 + ROI Diameter:12 + Reference Radius:3 + Reject High Decay Peak Pixels:false + Reject Low Decay Peak Pixels:false + Reverse Gates:false + SYNC Period:1.00000000000000E-8 + Save Phasor Graph:false + Save Phasor Plot:false + Saved Image File Format:"PNG" + Selected Image ROI(s):false + Selected Phasor Plot ROI(s):false + Shift Decay:false + Show Color Map Vertices:false + Show Last Decay Only:false + Show References:false + Smoothen Decay:false + Smoothing Parameter:-1 + Square Pulse Options:{"Method":"Mode","# Bins":100,"Threshold %":10,"Center on Midpoint":true,"Smoothen Plot":false} + Straighten Decay:false + Tail Fraction:0.5 + Time Step:1 + Use Backup Calibration if needed:false + Use Cursors:false + Use Decay Pre-Processing:false + Use File Timestamp:true + Use Fixed ROI Diameter:false + Use Guess Parameters:false + Use Image Brightness:false + Use Image Histogram for Contrast:true + Use Intensity Correction File:false + Use Last Fitted Parameters:false + Use Periodicity:false + Use Phasor Brightness:false + Use Phasor Plot Binning:false + User-defined Gate:{"X Array":[],"Y Array":[]} + User-defined IRF:{"X Array":[],"Y Array":[]} + Verbose Decay Pre-Processing:true + Verbose Error Mode:true + Verbose Mode:false + Verbose Settings Mode:false + Visible Settings Tab:"" + Wavelet Analysis Options:{"Wavelet":"db02","Option for Approximate Coefficients":"Threshold","# Levels":-1,"Threshold Settings":{"Thresholding Rule":"Universal","Rescaling Method":"Single level","Soft Threshold?":true},"Wavelet Transform Type":"Discrete"} + [0-1]-Normalize Decay:false AlliGator exposed internal variables JSON strings with values ------------------------------------------------------------- + Source Image Display Range:{"Min Value":-Infinity,"Max Value":Infinity} + Source Image Palette Information:{"Palette Name [# Colors,int?,inv?]":"Grayscale","Palette Type":5,"Palette Colors":[0,65793,131586,197379,263172,328965,394758,460551,526344,592137,657930,723723,789516,855309,921102,986895,1052688,1118481,1184274,1250067,1315860,1381653,1447446,1513239,1579032,1644825,1710618,1776411,1842204,1907997,1973790,2039583,2105376,2171169,2236962,2302755,2368548,2434341,2500134,2565927,2631720,2697513,2763306,2829099,2894892,2960685,3026478,3092271,3158064,3223857,3289650,3355443,3421236,3487029,3552822,3618615,3684408,3750201,3815994,3881787,3947580,4013373,4079166,4144959,4210752,4276545,4342338,4408131,4473924,4539717,4605510,4671303,4737096,4802889,4868682,4934475,5000268,5066061,5131854,5197647,5263440,5329233,5395026,5460819,5526612,5592405,5658198,5723991,5789784,5855577,5921370,5987163,6052956,6118749,6184542,6250335,6316128,6381921,6447714,6513507,6579300,6645093,6710886,6776679,6842472,6908265,6974058,7039851,7105644,7171437,7237230,7303023,7368816,7434609,7500402,7566195,7631988,7697781,7763574,7829367,7895160,7960953,8026746,8092539,8158332,8224125,8289918,8355711,8421504,8487297,8553090,8618883,8684676,8750469,8816262,8882055,8947848,9013641,9079434,9145227,9211020,9276813,9342606,9408399,9474192,9539985,9605778,9671571,9737364,9803157,9868950,9934743,10000536,10066329,10132122,10197915,10263708,10329501,10395294,10461087,10526880,10592673,10658466,10724259,10790052,10855845,10921638,10987431,11053224,11119017,11184810,11250603,11316396,11382189,11447982,11513775,11579568,11645361,11711154,11776947,11842740,11908533,11974326,12040119,12105912,12171705,12237498,12303291,12369084,12434877,12500670,12566463,12632256,12698049,12763842,12829635,12895428,12961221,13027014,13092807,13158600,13224393,13290186,13355979,13421772,13487565,13553358,13619151,13684944,13750737,13816530,13882323,13948116,14013909,14079702,14145495,14211288,14277081,14342874,14408667,14474460,14540253,14606046,14671839,14737632,14803425,14869218,14935011,15000804,15066597,15132390,15198183,15263976,15329769,15395562,15461355,15527148,15592941,15658734,15724527,15790320,15856113,15921906,15987699,16053492,16119285,16185078,16250871,16316664,16382457,16448250,16514043,16579836,16645629,16711422,16777215],"Interpolate?":false} + Time Gate Slider:0 + Type of Displayed Image:"Sum of All Gates" AlliGator exposed internal data JSON strings with values -------------------------------------------------------- + Calibration Phasor:{"m":0,"phi":0,"Phasor Frequency":0,"Reference Lifetime":0} + Phasor Calibration Map:{"m Array":[],"phi Array":[],"Phasor Frequency":80000000,"Calibration Lifetime":1.00000000000000E-9,"ROI Centers":[],"ROI Descriptors":[],"Map Resolution X":0,"Map Resolution Y":0} + ROIs:[] + Reference Decay:{"t Array":[],"I Array":[]} + Reference Decay Plot Name:"" + IRF Array:[] + IRF Array Time Axis:[]